TB-500 10 mg lyophilised peptide vial with blue flip-off cap and white label

NovaPep

Buy TB-500 Australia: TB-500 10mg Research Vial

$79AUD, GST included

In stock in Australia, batch certificate ready

Size: 10 mg vial

Certificate of analysis published for every batch
Third-party HPLC purity and MS identity testing
Dispatched same day before 3pm cut-off, tracked Australia-wide
Research use only, sold to adults aged 18 and over

Buy TB-500 online in Australia as a 10 mg research vial, tested for identity and purity on every lot. TB-500 is the acetylated heptapeptide Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln, the actin-binding motif sitting at positions 17 to 23 of thymosin beta-4, rather than the complete 43-residue protein. Laboratories use it to test whether that short motif alone affects cell migration and actin turnover. The identity data are C38H68N10O14, 889.0 g/mol and CAS 885340-08-9, and the mass reported on each certificate tells you immediately which molecule is in the vial. We supply it online in Australia as one lyophilised 10 mg vial, dispatched from stock held locally and backed by a lot certificate that is already public. Mass spectrometry and HPLC are run on every lot before it is stocked. The TB-500 calculator works out the concentration for whatever diluent volume you choose. To compare two repair pathways, look at BPC-157, the gastric pentadecapeptide researched for growth factor signalling. The vial contains powder only, so add bacteriostatic water if you need it. Keep it sealed at -20 °C, dry and out of the light, and refrigerate the solution after mixing. NovaPep supplies it as a research reagent to buyers aged 18 years or older.

For laboratory research use only. Not for human or veterinary use. Not TGA approved. See the NovaPep research use policy.

What Is TB-500?

TB 500 is a short synthetic chain of seven amino acids that copies the region of thymosin beta-4 that holds on to actin, the protein cells build into filaments each time they move, divide or reshape themselves. Picture it as the business end of a far bigger tool, separated and manufactured alone so laboratories can test whether that end works without the rest. You can order TB-500 online in Australia as a 10 mg research vial, and every lot ships with its own certificate.

Allan Goldstein's group first isolated thymosin beta-4 from calf thymus, and it was later found in nearly every cell type, where it keeps monomeric G-actin in reserve. Mapping work linked a large share of that actin-binding activity to the short LKKTETQ segment. TB-500 is a synthetic version of that motif, finished with an acetyl cap that protects it from aminopeptidases.

Parent protein is thymosin beta-4, 43 residues long, encoded by the gene TMSB4X.
The fragment covers positions 17 to 23 and is acetylated at the N-terminus in our vial.
Common assays include scratch closure, transwell migration, tube formation and aortic ring sprouting.
It should not be mixed up with Ac-SDKP, a different four-residue piece released from the N-terminus of the same protein and researched in fibrosis.

How TB-500 Works on Actin

Inside a cell, actin takes two forms. Free single units, known as G-actin, drift in the cytoplasm, while filaments, known as F-actin, assemble when those units join up at the front edge of a migrating cell. Thymosin beta-4 binds the free units in a one to one ratio and holds them in reserve, keeping a supply ready without allowing filaments to grow unchecked. When a cell has to travel toward a scratch or the edge of a wound, the reserve is freed and filaments lengthen rapidly. The LKKTETQ segment is where that binding happens, and an almost identical LKKTET motif appears in the WH2 domains of other actin regulating proteins, showing how important the sequence is for actin control across many proteins.

TB-500 Research: What Studies Have Looked At

The strongest sign that the motif is active by itself comes from Deborah Philp, Hynda Kleinman and co-workers. In 2003 they reported in The FASEB Journal that the actin-binding heptapeptide performed as well as full-length thymosin beta-4 in endothelial cell migration and aortic ring sprouting assays, whereas versions lacking part of the motif had no activity. This was cell and tissue explant research rather than an animal efficacy trial, yet it is the study that makes the fragment worth investigating in the first place. Labs planning similar studies can order TB-500 online in Australia, with identity and purity confirmed on every lot.

It is used to test a precise question. How much of the parent protein's influence on cell migration does the seven-residue motif deliver alone? That remains unresolved, because most published studies used full-length thymosin beta-4. TB-500 is a research reagent, it is not a medicine and it has no TGA approval. See the recovery peptides collection for related compounds.

The error we come across most often is treating TB500 peptide and thymosin beta-4 as one compound sharing one body of literature, and then applying a cardiac or corneal paper to the vial. Usually that does not hold. Choose assays in which the link to actin can be measured directly, include a scrambled heptapeptide as a negative control, and the fragment's own results will be robust. Our full guide to the TB-500 fragment sets out the protein-level studies that are frequently borrowed.

Thymosin Beta-4 and Australian Sport

Many Australians recognise thymosin beta-4 for a different reason. It was the substance at the heart of the Essendon Football Club supplements case, where in January 2016 the Court of Arbitration for Sport found 34 players guilty of anti-doping violations. WADA places thymosin beta-4 and its derivatives, TB-500 among them, under S2 (peptide hormones, growth factors and related substances), banned at all times, and Sport Integrity Australia enforces that list locally.

TB-500 Specifications

Product name	TB-500 (acetylated thymosin beta-4 fragment 17-23)
Size	10 mg lyophilised vial
Sequence	Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ)
Peptide length	7 amino acids, N-terminally acetylated
Formula	C38H68N10O14
Molar mass	889.0 g/mol
CAS	885340-08-9
Presentation	White freeze-dried cake in a sealed 3 mL glass vial with a blue flip-off cap
Quality testing	Mass spectrometry for identity and HPLC for purity, with a certificate published for each lot
In the box	One vial of 10 mg TB-500 powder per unit, with bacteriostatic water sold as a separate item
Storage conditions	Sealed at -20 °C away from light and moisture, then 2 to 8 °C after reconstitution
Stock status	In stock
Intended use	In vitro laboratory research only

With TB-500 from NovaPep you know precisely what is in the vial. Across the market the name gets applied loosely, but here it always refers to the 889 g/mol heptapeptide, confirmed for every lot. Offering one fill size keeps ordering easy and the documentation brief.

A TB-500 unit is a single 3 mL glass vial with 10 mg of white lyophilised powder and no other contents, so there is no diluent, no carrier peptide and no second compound. The heptapeptide is also one ingredient in three premixed vials sold on this site, the Wolverine Blend, KLOW and GLOW, but this page offers the fragment alone for studies that need it measured in isolation.

Lysine and threonine make up much of the heptapeptide, which makes it highly water soluble, and the acetyl group on its N-terminus adds 42 mass units to the unmodified chain. These points are relevant on the certificate, where the measured mass should be near 889 g/mol and nowhere near the approximately 4963 g/mol of full-length thymosin beta-4.

Check the HPLC trace and purity value on the same report before you purchase. Reports are sorted by lot number in our certificate library, so read yours first and then match the code on the label once the vial is delivered.

The same step strengthens your publication. Before comparing your results with a published study, check which molecule the authors really used, because a lot of thymosin research relied on the full recombinant or synthetic protein instead of the fragment. When a lab notes the lot code, measured mass and HPLC result in its records, it can show reviewers exactly what was added to each well and each animal group. That documentation is the best argument for buying from a supplier that publishes its certificates rather than just describing them.

Handling and Storage of TB-500

Add bacteriostatic water if your stock of diluent is running low. Let the vial warm to room temperature, run a measured volume down the glass wall and roll it gently until it clears, avoiding any foam. Adding 2.5 mL to the 10 mg vial gives 4 mg/mL, and the TB-500 concentration calculator handles any other volume. Order TB-500 online in Australia and it travels by tracked Australia Post from our local stock.

Store unopened vials frozen at -20 °C away from light. Keep the solution in the fridge at 2 to 8 °C, preferably split into small aliquots so only one portion is thawed at a time.

TB-500 Compared With BPC-157

Choose TB-500 when you are studying the cytoskeleton, actin turnover and the way cells move into a wound edge in culture. Choose BPC-157, sold in a 10 mg vial, when the focus is growth factor signalling, nitric oxide or models of the gut lining. Separate vials let you control each amount and run single-compound controls. Our BPC-157 vs TB-500 comparison looks at both mechanisms in greater detail.

If your protocol has already fixed equal amounts of both, the Wolverine Blend combines 5 mg of each in one vial.

Other Names for TB 500 Peptide

Labelling differs between suppliers, and you will see TB-500, TB 500, TB500 and thymosin beta-4 fragment, along with the sequence forms Ac-LKKTETQ and acetyl-LKKTETQ-OH. Some catalogues use thymosin beta-4 (17-23) or the Tb4 actin-binding fragment as well. All of them describe the acetylated seven-residue chain registered as CAS 885340-08-9.

Full-length thymosin beta-4, which is also known by its gene name TMSB4X, is a separate and far larger molecule. The fragment does not work through a receptor, and the terms used around it focus on G-actin sequestration, lamellipodia and cell motility.

Buy TB-500 Online in Australia

There is one fill to choose, the 10 mg vial. That amount is enough for a standard cell migration series or a rodent study plan, and since reconstituted peptide should be used within a few weeks, a moderate fill reduces waste if work is put on hold.

Labs chasing the best TB500 peptide should compare lot certificates, because the acetylated heptapeptide is easy to confuse with full thymosin beta-4.

When you buy several vials in a single checkout they are drawn from the same lot wherever stock permits, so a single certificate covers every run in your series. TB 500 can only be purchased by adults aged 18 or over, who confirm their age and laboratory purpose before paying.

You will not find a counter to visit or a click-and-collect option. TB-500 is ordered online on this page and sent from our Australian stock to any address in the city or the regions, including laboratories buying research peptides in Perth. Orders placed by 3pm on a business day leave the same afternoon, and you can follow them on the order tracking page.

How Australian Regulations Treat TB-500

Regulators handle it much more strictly than its size might imply. After a referral to the Advisory Committee on Medicines Scheduling, the TGA placed thymosin beta-4 and TB-500 in the Poisons Standard as Schedule 4 (prescription only) substances. As of October 2026 the TGA has approved no medicine containing either, and none is registered on the ARTG. Healthy Male, the Australian men's health organisation, points out that a "for research only" label does not make possession or use lawful without a prescription. For this reason TB-500 in Australia is offered almost only by research suppliers, since there is no registered product to sell through any other route.

If you intend to buy TB500 for laboratory work, NovaPep keeps the 10 mg vial in Australian stock as a research reagent for in vitro use, sold only to adults 18 and over and never for human or veterinary use. The TB-500 background guide explains where the fragment came from and the papers that support it.

TB-500 at a Glance

An actin-binding seven-residue peptide

TB-500 reproduces the small region of thymosin beta-4 that holds actin, the scaffold protein cells rely on to move. It is a synthetic heptapeptide with an acetyl cap.

Buying the 10 mg vial

Order a single sealed 10 mg vial of dry powder. It contains no liquid, so add bacteriostatic water to your cart separately.

Studying cell movement

Researchers use it in cell culture and animal models to examine cell migration and how tissue reorganises after damage. These are research mechanisms and not medical applications.

Different from BPC-157

BPC-157 is double the length, is derived from a stomach protein and works through separate pathways. Complete thymosin beta-4 is six times the length of TB-500.

Storage once it arrives

Keep the powder in the freezer and the solution in the fridge, both protected from light. Splitting into aliquots prevents the repeated freeze and thaw cycles that degrade a peptide.

A verifiable mass

HPLC and mass spectrometry are run on each lot, and a result near 889 g/mol confirms the fragment. Certificates can be viewed before you buy.

TB-500 FAQ

What is TB-500 and how does it work?

TB-500 is a synthetic copy of the LKKTETQ segment of thymosin beta-4, capped with an acetyl group. That segment is where the parent protein binds free G-actin and holds it in reserve. Cells draw on that reserve to build filaments when they migrate, so the fragment is studied in cell movement assays.

Are TB-500 and thymosin beta-4 the same thing?

No. Thymosin beta-4 is a protein of 43 amino acids weighing about 4963 g/mol, whereas our TB-500 is the acetylated 17 to 23 fragment at 889 g/mol. The mass on the certificate distinguishes them straight away, which is important when comparing your data with studies that used the whole protein.

How do laboratories use TB-500?

It lets researchers isolate the actin-binding motif of thymosin beta-4. It appears in scratch assays, endothelial and keratinocyte migration work and rodent tissue models, where labs test whether the short fragment repeats effects described for the full protein. It is sold only for in vitro and laboratory research.

How does TB-500 differ from BPC-157?

TB-500 suits questions about actin, the cytoskeleton and cell migration, while BPC-157 suits work on growth factors, nitric oxide or the gut lining. The two molecules are not related, so many laboratories run them as separate vials, each with its own controls. If a protocol is set at equal amounts, the Wolverine Blend is an option.

Can I buy TB 500 peptide near me?

You order it online from this page, as NovaPep has no shop or collection point anywhere. The identical 10 mg vial with its published lot certificate goes to labs in Perth, Cairns or Wagga Wagga alike, sent by tracked delivery from local stock, with timeframes shown on our delivery information page.

What is the process to buy TB-500 online from Australian stock?

Add the 10 mg vial to your cart and complete checkout on this page. It ships from our stock in Australia and leaves the same afternoon if you order before 3pm on a business day. Add bacteriostatic water if you need a diluent, and confirm during checkout that you are aged 18 or over.